The preparation methods of silk fibroin peptide mainly include: salt solution, acid hydrolysis, alkaline hydrolysis, enzymatic hydrolysis and the like.

 

Degradation with salt solution can obtain silk fibroin with a molecular weight of tens of thousands, which is still a macromolecular protein and thus affects its attraction and nutritional value. The acid hydrolysis method can fully hydrolyze silk protein, but it destroys all tryptophan and serine. The tyrosine and threonine moieties are destroyed; the alkaline hydrolysis method is used to produce racemization, which reduces the nutritional value; the enzymatic hydrolysis method is the most effective method for degrading silk protein due to mild reaction conditions and small damage to amino acids. .

 

We need to adopt different methods to prepare silk fibroin peptides according to different actual needs, or combine them with various preparation methods to prepare silk fibroin peptides that meet the requirements.